[Human recombinant antibodies to variola virus].

Tikunova, N V; Bovshik, E I; Iun, T E; Zhirakovskaia, E V; Morozova, V V; Batanova, T A; Gus'kov, A A; Sokunova, E B; Il'ichev, A A; Sandakhchiev, L S

Tikunova, N V; Bovshik, E I; Iun, T E; Zhirakovskaia, E V; Morozova, V V; Batanova, T A; Gus'kov, A A; Sokunova, E B; Il'ichev, A A; Sandakhchiev, L S.

Vopr Virusol ; 50(6): 20-5, 2005.

Article en Ru | MEDLINE | ID: mdl-16408625

RESUMEN

Eight specific antibodies to live variola virus (VV), Ind-3a strain, and 7 antibodies to VV, Butler strain, were selected from the synthetic combinatorial phage display library on single-chain (scFv) human antibodies. Indirect solid-phase enzyme immunoassay showed the ability of these antibodies to bind the VV strains Ind-3a, Butler, Brazil-131, Kuw-5, and Congo-2. Moreover, earlier selected human scFv antibodies were also tested in the reaction of binding to the above VV strains. The experiments could reveal the antibodies that bound alastrim strains more effectively that did other VV strains. The nucleotide sequences encoding for the selected scFv antibodies were determined.

Asunto(s)

Anticuerpos Antivirales/inmunología; Virus de la Viruela/inmunología; Secuencia de Aminoácidos; Anticuerpos Antivirales/biosíntesis; Anticuerpos Antivirales/genética; Técnicas Químicas Combinatorias; Reacciones Cruzadas; Humanos; Región Variable de Inmunoglobulina/biosíntesis; Región Variable de Inmunoglobulina/genética; Región Variable de Inmunoglobulina/inmunología; Biblioteca de Péptidos; Proteínas Recombinantes/genética; Proteínas Recombinantes/inmunología

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Viruela / Anticuerpos Antivirales Límite: Humans Idioma: Ru Revista: Vopr Virusol Año: 2005 Tipo del documento: Article Pais de publicación: Rusia

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