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Identification of otubain 1 as a novel substrate for the Yersinia protein kinase using chemical genetics and mass spectrometry.
Juris, Stephen J; Shah, Kavita; Shokat, Kevan; Dixon, Jack E; Vacratsis, Panayiotis O.
Afiliación
  • Juris SJ; Department of Chemistry and Biochemistry, University of Windsor, 401 Sunset Avenue, Windsor, Ont., Canada N9B3P4.
FEBS Lett ; 580(1): 179-83, 2006 Jan 09.
Article en En | MEDLINE | ID: mdl-16364312
Yersinia encodes a protein kinase, YpkA, which disrupts the actin cytoskeleton. Using an approach termed chemical genetics, we identified a 36-kDa substrate for YpkA in both J774 lysates and bovine brain cytosol. Mass spectrometry analysis identified this substrate as FLJ20113, an open reading frame that corresponds to otubain 1, a deubiquitinating enzyme implicated in immune cell clonal anergy. We demonstrate that otubain 1 is phosphorylated by YpkA in vitro and interacts with YpkA and actin in vivo. Identification of otubain 1 as a YpkA substrate suggests that regulation of immune cell anergy may be a survival mechanism for Yersinia.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Yersinia / Cisteína Endopeptidasas / Proteínas Serina-Treonina Quinasas Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: FEBS Lett Año: 2006 Tipo del documento: Article Pais de publicación: Reino Unido
Buscar en Google
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Yersinia / Cisteína Endopeptidasas / Proteínas Serina-Treonina Quinasas Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: FEBS Lett Año: 2006 Tipo del documento: Article Pais de publicación: Reino Unido