Further arguments in favour of direct covalent binding of Ochratoxin A (OTA) after metabolic biotransformation.
Food Addit Contam
; 22 Suppl 1: 75-87, 2005.
Article
en En
| MEDLINE
| ID: mdl-16332625
Ochratoxin A (OTA) is nephrotoxic to all animal species, carcinogenic for rats and mice and probably implicated in human Balkan endemic nephropathy and the associated urothelial tract tumour. Controversial results concerning genotoxicity and biotransformation of OTA have been generated. By (32)P post-labelling technique, a dose- and time-dependent DNA adduct formation is observed in vivo and in vitro. Use of several inducers or inhibitors of biotransforming enzymes (including cytochrome P 450, cyclooxygenase, lipoxygenase, glutathione-S-transferase), demonstrated that OTA is biotransformed into genotoxic derivatives damaging for DNA. Authentic C8dG-OTA standards have been synthesized by photo-oxidation. Both of them (C-C8 & O-C8) co-migrate on TLC with two adducts formed by in vitro incubation of OTA in the presence of kidney microsomes, and in vivo in kidney of pig or rodent fed OTA as well as in kidney and bladder tumour of humans exposed to OTA. Several OTA metabolites have been isolated from tissues or cells treated by OTA. The open ring lactone (OP-OTA) and quinone OTA (OTQ) are genotoxic.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ocratoxinas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Food Addit Contam
Asunto de la revista:
CIENCIAS DA NUTRICAO
Año:
2005
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido