Sterilisation of extracted human teeth for educational use.
Indian J Med Microbiol
; 23(4): 256-8, 2005 Oct.
Article
en En
| MEDLINE
| ID: mdl-16327123
Sixty intact, non-carious and unrestored teeth extracted due to periodontal disease were used to determine the most effective method of sterilisation. The teeth were divided into six groups, each containing 10 teeth. Group 1 teeth were immersed in 10% formalin for seven days, group 2 teeth were immersed in 3% hydrogen peroxide for seven days, group 3 teeth were immersed in 2.6% sodium hypochlorite for seven days, group 4 teeth were boiled in water at 100 degrees C for 20 minutes, group 5 teeth were autoclaved at 121 degrees C at 15 lbs psi for 30 minutes, and group 6 teeth were immersed in normal saline for seven days. After the treatment, the teeth were individually inoculated into trypticase soy broth and incubated for 48 hours. A questionnaire survey was also conducted to determine the awareness of dental students regarding infection due to extracted human teeth and the common disinfection/sterilisation methods used. Autoclaving at 121 degrees C, 15 lbs psi for 30 minutes and immersion in 10% formalin for seven days were effective in disinfecting/sterilising extracted human teeth. Chemicals such as 2.6% sodium hypochlorite, 3% hydrogen peroxide and boiling in water were not effective. The results indicate that autoclaving for 30 minutes or immersion in 10% formalin for seven days could be effectively used for disinfection/sterilisation of extracted human teeth.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Diente
/
Esterilización
/
Educación en Odontología
Límite:
Humans
Idioma:
En
Revista:
Indian J Med Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
Estados Unidos