Analysis of the matrix protein gene sequence of the Asian lineage of peste-des-petits ruminants vaccine virus.
Vet Microbiol
; 113(1-2): 83-7, 2006 Mar 10.
Article
en En
| MEDLINE
| ID: mdl-16297575
The M gene nucleotide sequence of an Indian peste-des-petits ruminants (PPRV) vaccine virus ("PPRV Sungri/96") belonging to Asian lineage was determined. The gene is 1476 nucleotides long with a single open reading frame (ORF). The nucleotide and predicted amino acid sequence was compared with the homologous region of the African Lineage Vaccine virus "PPRV/Nigeria/75/1". The nucleotide sequence of the "PPRV Sungri/96" was 86% identical to that of "PPRV/Nigeria/75/1", while a homology of 93% and 95% could be observed in the ORF and amino acids level, respectively. The M gene encodes a protein of 335 amino acids, with a predicted molecular weight (MW) of 37.8 kDa. The ORF is flanked by a 3' untranslated region of 436 nucleotides and a high level of sequence divergence (approximately 30%) could be observed in this region between the vaccine viruses of Asian and African lineages. A high degree of conservation of several amino acids of this protein observed previously was also confirmed in this study.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vacunas Virales
/
Proteínas de la Matriz Viral
/
Virus de la Peste de los Pequeños Rumiantes
/
Peste de los Pequeños Rumiantes
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Vet Microbiol
Año:
2006
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
Países Bajos