Surface plamon resonance imaging of DNA based biosensors for potential applications in food analysis.
Biosens Bioelectron
; 21(6): 894-900, 2005 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-16257658
The adsorption processes of oligonucleotides immobilised onto suitable photolithographic patterned gold substrates have been investigated in aqueous buffer solution by using a home made surface plasmon resonance (SPR) imaging equipment. A rapid self-assembled method for the construction of DNA chips to be used in SPR imaging experiments have been followed. The immobilised DNA molecules (probes) adopted in our SPR experiments anchored to a gold surface via thiol group were 5'thiol-modified containing a (CH(2))(15) tail. The hybridisation processes taking place with its complementary sequence have been observed and characterized by monitoring phenomena by a SPR imaging system. The two analysed oligonucleotides (probes and target) are of interest in plant gene biotechnological application and differing for the presence at the 5'-end of a poly T16 spacer. Dynamic investigation of smallest changes in SPR imaging pictures performed in liquid phase in the presence of DNA complementary probes have been performed. Quantitative information in terms of threshold of sensitivity has been extracted by using a specific images treatment.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Técnicas Biosensibles
/
Contaminación de Alimentos
/
Resonancia por Plasmón de Superficie
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Análisis de Secuencia por Matrices de Oligonucleótidos
/
Análisis de los Alimentos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Reino Unido