A convenient method for the evaluation of anti-tumor agents affecting the cell cycle.
J Biosci Bioeng
; 90(5): 574-6, 2000.
Article
en En
| MEDLINE
| ID: mdl-16232914
Mitotic index (MI), defined as the ratio (%) of the mitotic-phase-arrested cell number to the total cell number, is higher in the presence of colcemid than in its absence. MI is reduced by anti-tumor agents used in conjunction with colcemid. The ratio of MI, which is defined by the dose of colcemid to that of the supplement with etoposide as an anti-tumor agent and is designated MIR, was 10% at an exposure of 2 h, suggesting that etoposide blocks the cell cycle before 2 h of the mitotic phase. Thus, we established a convenient method to identify a target point in the cell cycle for anti-tumor agents using colcemid. RBL-2H3, a mammalian cell line, exhibited a large number of 2-fold swollen nuclear envelopes after exposure to etoposide. The G2-index, which is the ratio of the number of swollen nuclei to the total number of nuclei, was higher when the cells were incubated with etoposide than without it. In this study, we suggest that the measurement of the G2-index contributes to the screening of putative anti-tumor agents.
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
J Biosci Bioeng
Asunto de la revista:
ENGENHARIA BIOMEDICA
/
MICROBIOLOGIA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Japón