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[Construction of infectious Japanese encephalitis virus clone based on the cDNA template of the attenuated live vaccine production strain SA14-14-2].
Zeng, Ming; Jia, Li-li; Yu, Yong-xin; Dong, Guan-mu; Liu, Wen-xue; Wang, Zhi-wei; Li, De-fu.
Afiliación
  • Zeng M; National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi ; 19(1): 9-11, 2005 Mar.
Article en Zh | MEDLINE | ID: mdl-16201461
OBJECTIVE: To construct infectious Japanese encephalitis virus (JEV) based on the in vitro-ligated cDNA template of the vaccine strain SA14-14-2, and identify the virus. METHODS: Full-length genomic cDNA of JEV SA14-14-2 strain was ligated and then RNA was transcribed in vitro, the infective virus was obtained by transfecting the RNA into Vero cells and identified. RESULTS: The infective clone of JEV was constructed, the virulence was weaker than the wild virus. CONCLUSION: It was possible to construct infectious clone from the production strain of live attenuated Japanese B encephalitis vaccine.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Viral / ADN Complementario / Virus de la Encefalitis Japonesa (Especie) Límite: Animals Idioma: Zh Revista: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi Asunto de la revista: VIROLOGIA Año: 2005 Tipo del documento: Article País de afiliación: China Pais de publicación: China
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Viral / ADN Complementario / Virus de la Encefalitis Japonesa (Especie) Límite: Animals Idioma: Zh Revista: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi Asunto de la revista: VIROLOGIA Año: 2005 Tipo del documento: Article País de afiliación: China Pais de publicación: China