Measurement of immunoglobulins G, A, and M levels in B-lymphocytes culture.

Grandjenette, Cindy; Kennel, Anne; Massin, Frédéric; Faure, Gilbert C; Béné, Marie C; Montagne, Paul

Grandjenette, Cindy; Kennel, Anne; Massin, Frédéric; Faure, Gilbert C; Béné, Marie C; Montagne, Paul.

Afiliación

Grandjenette C; GRIP, Immunology Laboratory, Faculty of Medecine, Vandoeuvre lés Nancy, France.

J Immunoassay Immunochem ; 26(4): 303-11, 2005.

Article en En | MEDLINE | ID: mdl-16153015

RESUMEN

Nephelometric immunoassays were developed for human IgG, IgA, and IgM quantitation in B-lymphocytes culture media. They allowed measurement of immunoglobulin (Ig) levels over a broad range of concentrations with good accuracy and precision. The kinetics of Ig production in B-lymphocyte cultures was followed and the mean amount of each Ig was determined in six different samples after three days of culture. The nephelometric immunoassays reported here could be used to study, in vitro, the influence of various molecules (inhibitory or amplifying effect) on B-lymphocytes' functional capacities.

Asunto(s)

Linfocitos B/química; Inmunoglobulina A/análisis; Inmunoglobulina G/análisis; Inmunoglobulina M/análisis; Células Cultivadas; Niño; Preescolar; Medios de Cultivo/análisis; Humanos; Inmunoensayo/métodos; Lactante; Nefelometría y Turbidimetría/métodos; Tonsila Palatina/citología; Reproducibilidad de los Resultados

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina A / Inmunoglobulina G / Inmunoglobulina M / Linfocitos B Límite: Child / Child, preschool / Humans / Infant Idioma: En Revista: J Immunoassay Immunochem Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2005 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido

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