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Identification of a new virulence factor, BvfA, in Brucella suis.
Lavigne, Jean-Philippe; Patey, Gilles; Sangari, Felix J; Bourg, Gisèle; Ramuz, Michel; O'Callaghan, David; Michaux-Charachon, Sylvie.
Afiliación
  • Lavigne JP; Institut National de la Santé et de la Recherche Médicale, U431, Faculté de Médecine, Avenue Kennedy, 30900 Nimes, France.
Infect Immun ; 73(9): 5524-9, 2005 Sep.
Article en En | MEDLINE | ID: mdl-16113268
We report the identification of BvfA (for Brucella virulence factor A), a small periplasmic protein unique to the genus Brucella, which is essential for the virulence of Brucella suis. A BvfA knockout mutant was highly attenuated both in in vitro macrophage infection assays and in vivo in the murine model of brucellosis. Fluorescence-activated cell sorting analysis with green fluorescent protein fusions showed that the expression of bvfA is induced within macrophages by phagosome acidification and coregulated with the B. suis virB operon, suggesting that it too may play a role in the establishment of the intracellular replication niche.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Brucella suis Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Infect Immun Año: 2005 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Brucella suis Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Infect Immun Año: 2005 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos