[Identification of genes that are specifically/preferentially expressed in developing cotton fibers by mRNA fluorescence differential display (FDD)].
Sheng Wu Gong Cheng Xue Bao
; 20(1): 39-42, 2004 Jan.
Article
en Zh
| MEDLINE
| ID: mdl-16108487
Fluorescence differential display (FDD) technique was used to identify genes that are specifically or preferentially expressed in different developmental stages of cotton fiber cells. One hundred and nine differentially displayed cDNA fragments were isolated using 9, 21 and 27 DPA (days postanthesis) fibers as experimental materials. By a combination of two rounds of reverse Northern hybridization and Northern blot analyses, a number of such cDNA fragments were proved to represent fiber-specific/preferential genes. Sequencing determination and database searching indicated that most of these genes are novel. This work is an important step towards cloning the full-length cDNAs and characterizing the cellular functions of aforementioned genes in fiber development.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Gossypium
/
Perfilación de la Expresión Génica
/
Fibra de Algodón
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Sheng Wu Gong Cheng Xue Bao
Asunto de la revista:
BIOTECNOLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China