Acetylcholine stimulates cyclic ADP-ribose formation via M1 muscarinic receptors in rat superior cervical ganglion.
Biochem Biophys Res Commun
; 335(3): 920-4, 2005 Sep 30.
Article
en En
| MEDLINE
| ID: mdl-16105661
The role of cyclic ADP-ribose (cADPR) as the downstream signal of neuronal muscarinic acetylcholine receptors (mAChRs) and the enzyme responsible for its synthesis, ADP-ribosyl cyclase, were examined in the rat superior cervical ganglion (SCG). Application of acetylcholine or other mAChR agonists increased the ADP-ribosyl cyclase activity by about 250-300% in crude membrane fractions from the SCG of 14-day-old rats. This effect was inhibited by atropine or by the M1-mAChR antagonist, pirenzepine, and was mimicked by GTP. These results indicate that the M1 mAChRs couple to the membrane-bound form of ADP-ribosyl cyclase and suggest that cADPR is a second messenger of M1 mAChR signaling in nervous tissue.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acetilcolina
/
ADP-Ribosa Cíclica
/
Receptor Muscarínico M1
/
Ganglios
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2005
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Estados Unidos