Identification of genetically modified potato (Solanum tuberosum) cultivars using event specific polymerase chain reaction.
J Agric Food Chem
; 53(17): 6691-6, 2005 Aug 24.
Article
en En
| MEDLINE
| ID: mdl-16104786
Several genetically modified (GM) cultivars are registered in Canada although they are not currently in commercial production. The GM cultivars can be distinguished from the non-GM and other GM cultivars by analyzing the DNA nucleotide sequence at the insertion site of the transgene corresponding to a single transformation event in the plant genome. Techniques based on modified polymerase chain reaction (PCR) strategies were used to generate sequence information from the plant genome flanking the insertion site of transgenic DNA for specific GM potato events. The plant genome sequence adjacent to the transgenic insertion was used to design PCR primers, which could be used in combination with a primer annealing to one of the nearby inserted genetic elements to amplify an event specific DNA fragment. The event specific PCR fragments generated were sequenced to confirm the specificity of the method.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Solanum tuberosum
/
Reacción en Cadena de la Polimerasa
/
Plantas Modificadas Genéticamente
/
ADN de Plantas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Agric Food Chem
Año:
2005
Tipo del documento:
Article
País de afiliación:
Canadá
Pais de publicación:
Estados Unidos