[Protective immunity induced by multivalent DNA vaccine of Schistosoma japonicum Mr23 x 10(3) membrane antigen and IL-12 in mice].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
; 23(2): 82-5, 2005 Apr 30.
Article
en Zh
| MEDLINE
| ID: mdl-16042171
OBJECTIVE: To develop multivalent DNA vaccine PV-IL12-Sj23 which co-expresses Sj23 and cytokine IL-12, and investigate its protective efficacy in BALB/c mice against challenge infection. METHODS: On the basis of the reconstructed plasmid PV-IL12-Sj23 and plasmid PV-IL12, blank plasmid PV and plasmid PV-Sj23 only expressing Sj23 were constructed. Fifty BALB/c male mice were divided into five groups, which were immunized intramuscularly with multivalent DNA vaccine PV-IL12-Sj23, plasmid PV-Sj23 expressing Sj23, plasmid PV-IL12 expressing cytokine IL-12, blank plasmid PV and saline, respectively. Each mouse was immunized with 100 microg DNA only once. All the mice were challenged with 40 cercariae at week 4, killed and perfused for collection of worms at week10. The number of recovered worms and eggs in the liver were counted. RESULTS: Blank plasmid PV and plasmid PV-Sj23 expressing Sj23 were successfully constructed. The worm reduction rate in PV-IL12-Sj23 group and PV-Sj23 group was 45.5% and 27.2% (P< 0.05) respectively. The number of eggs in liver tissue was reduced by 58.4% and 33.9% respectively. CONCLUSION: Multivalent DNA vaccine PV-IL12-Sj23 can induce protective immunity against Schistosoma japonicum in BALB/c mice significantly, with a better protective efficacy than the monovalent DNA vaccine PV-Sj23.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Schistosoma japonicum
/
Esquistosomiasis Japónica
/
Proteínas del Helminto
/
Interleucina-12
/
Vacunas de ADN
/
Proteínas de la Membrana
/
Antígenos Helmínticos
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
Asunto de la revista:
PARASITOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China