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Association between beta2-glycoprotein I gene polymorphisms and pediatric SLE and antiphospholipid antibodies.
von Scheven, E; Elder, M E.
Afiliación
  • von Scheven E; Pediatric Rheumatology, University of California, San Francisco, CA 94143, USA. evonsche@peds.ucsf.edu
Lupus ; 14(6): 440-4, 2005.
Article en En | MEDLINE | ID: mdl-16038107
Antibodies against phospholipids (PL) and PL-binding proteins have been causally implicated in antiphospholipid syndrome (APS). Mutations in the fifth domain of the beta2-glycoprotein I (beta2GPI) protein, a putative PL-binding site, may play a critical role in APS pathogenesis. The purpose of this study was to identify associations between beta2GPI mutations and both antiphospholipid antibodies (aPL) and their associated clinical manifestations in a pediatric and adolescent cohort and to search for novel mutations. Genetic analysis of beta2GPI was performed in 58 youths with systemic lupus erythematosus (SLE) and/or aPL, to identify known polymorphisms at amino acids 247 and 306 as well as novel mutations in exon 7 of the beta2GPI gene, and their association with aPL-associated clinical manifestations. Our results demonstrate an association between substitution of Val for Leu at AA247 (L247V) of beta2GPI and both the development of aPL (P = 0.05) and aPL-associated clinical manifestations (P = 0.03) among pediatric patients. The odds ratio associated with risk of aPL-associated clinical manifestations for the homozygous VV polymorphism was 5.5 (CI 1.3-23, P = 0.03) for the overall cohort, and 4.75 (CI 0.66-55.49, P = 0.06) after adjusting for ethnicity. The association was not significant after stratifying for SLE versus non-SLE. Association between the VV genotype at amino acid 247 of beta2GPI and clinical disease supports a genetic cause for APS among children and adolescents. Neither novel exon 7 beta2GPI mutations or the previously described C306G polymorphism was identified in this pediatric cohort.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas / Anticuerpos Antifosfolípidos / Lupus Eritematoso Sistémico Tipo de estudio: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Adolescent / Adult / Child / Child, preschool / Female / Humans / Male Idioma: En Revista: Lupus Asunto de la revista: REUMATOLOGIA Año: 2005 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas / Anticuerpos Antifosfolípidos / Lupus Eritematoso Sistémico Tipo de estudio: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Adolescent / Adult / Child / Child, preschool / Female / Humans / Male Idioma: En Revista: Lupus Asunto de la revista: REUMATOLOGIA Año: 2005 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido