Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane.
Tissue Eng
; 11(5-6): 709-14, 2005.
Article
en En
| MEDLINE
| ID: mdl-15998212
Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ingeniería de Tejidos
/
Células de la Granulosa
/
Membranas Artificiales
/
Modelos Biológicos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Tissue Eng
Asunto de la revista:
BIOTECNOLOGIA
/
HISTOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Estados Unidos