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Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane.
Vigo, D; Villani, S; Faustini, M; Accorsi, P A; Galeati, G; Spinaci, M; Munari, E; Russo, V; Asti, A; Conte, U; Torre, M L.
Afiliación
  • Vigo D; Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Milan, Italy.
Tissue Eng ; 11(5-6): 709-14, 2005.
Article en En | MEDLINE | ID: mdl-15998212
Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Células de la Granulosa / Membranas Artificiales / Modelos Biológicos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Tissue Eng Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2005 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Células de la Granulosa / Membranas Artificiales / Modelos Biológicos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Tissue Eng Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2005 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos