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Mononuclear cell subsets in bronchoalveolar lavage fluid during Dictyocaulus viviparus infection of calves: a potential role for gamma/delta TCR-expressing cells in airway immune responses?
Hagberg, M; Wattrang, E; Niskanen, R; Tråvén, M; Höglund, J; Lundén, A.
Afiliación
  • Hagberg M; Department of Parasitology (SWEPAR), National Veterinary Institute and Swedish University of Agricultural Sciences, Uppsala, Sweden. malin.hagberg@sva.se
Parasite Immunol ; 27(5): 151-61, 2005 May.
Article en En | MEDLINE | ID: mdl-15987338
Mononuclear cell populations in the lungs of calves infected with Dictyocaulus viviparus were studied during primary infection and reinfection in order to identify cells involved in development of protective immunity to parasitic bronchitis. Three groups of calves were either inoculated with 500 third-stage larvae at both weeks 0 and 10 (n = 6), inoculated only at week 10 (n = 6), or remained uninfected (n = 3). The animals were monitored weekly by collection of bronchoalveolar lavage fluid (BALF), blood and faeces. Among mononuclear BALF-cell populations, the gamma/delta TCR-expressing cells showed a pronounced transient increase in proportion as well as in relative cell size 2 weeks post primary infection, whereas CD4-, CD8-, Ig- and CD14-expressing cells showed no significant differences related to the infection. The increase in gamma/delta TCR-expressing cells coincided with significantly increased proportions of eosinophils and recovery of adult worms in BALF. After reinfection, gamma/delta TCR-expressing cells increased again, but not until week 3 post inoculation, whereas eosinophils were increased by week 2 and reached higher levels than after primary infection. After reinfection, establishment of D. viviparus was less successful than after primary infection. In conclusion, these results indicate a role for gamma/delta TCR-expressing lymphocytes in the pathogenesis of D. viviparus infection.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Líquido del Lavado Bronquioalveolar / Subgrupos de Linfocitos T / Receptores de Antígenos de Linfocitos T gamma-delta / Infecciones por Dictyocaulus / Dictyocaulus / Enfermedades Pulmonares Parasitarias Límite: Animals Idioma: En Revista: Parasite Immunol Año: 2005 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Líquido del Lavado Bronquioalveolar / Subgrupos de Linfocitos T / Receptores de Antígenos de Linfocitos T gamma-delta / Infecciones por Dictyocaulus / Dictyocaulus / Enfermedades Pulmonares Parasitarias Límite: Animals Idioma: En Revista: Parasite Immunol Año: 2005 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido