Role of divalent cations in the subunit associations of complex flagella from Rhizobium meliloti.
J Bacteriol
; 174(12): 3896-902, 1992 Jun.
Article
en En
| MEDLINE
| ID: mdl-1597412
Rhizobium meliloti, a symbiotic, nitrogen-fixing soil bacterium with complex flagella, as well as other members of the family Rhizobiaceae, rapidly lost motility when suspended in buffers lacking divalent cations but retained good motility in buffers containing calcium, magnesium, barium, or strontium. Loss of motility was associated with loss of flagella from the cells. Analysis of flagella by sedimentation, gel electrophoresis, and electron microscopy revealed that removal of divalent cations from the complex flagella of R. meliloti resulted in extensive dissociation of the flagellar filaments into low-molecular-weight subunits. Accordingly, divalent cations such as calcium and magnesium that are normally present at high concentrations in the soil solution may be crucial to the assembly and rigidity of complex flagella.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cationes Bivalentes
/
Sinorhizobium meliloti
/
Flagelos
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
J Bacteriol
Año:
1992
Tipo del documento:
Article
Pais de publicación:
Estados Unidos