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Localization of alpha-dystroglycan on the podocyte: from top to toe.
Vogtländer, Nils P J; Dijkman, Henry; Bakker, Marinka A H; Campbell, Kevin P; van der Vlag, Johan; Berden, Jo H M.
Afiliación
  • Vogtländer NP; Division of Nephrology (545), Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands.
J Histochem Cytochem ; 53(11): 1345-53, 2005 Nov.
Article en En | MEDLINE | ID: mdl-15956031
alpha-Dystroglycan (DG) is a negatively charged membrane-associated glycoprotein that links the cytoskeleton to the extracellular matrix. Previously, we described that alpha-DG covers the whole podocyte cell membrane in the rat. However, our finding was challenged by the description of a strictly basolateral localization in human kidney biopsies, using a different antibody against alpha-DG. Therefore, we studied the exact localization of glomerular alpha-DG by using these two antibodies in both species. The studies were performed by using monoclonal antibodies (MoAbs) IIH6 and VIA4.1 in immunofluorescence, confocal microscopy, and immunoelectron microscopy on both rat and human kidney sections, as well as on cultured mouse podocytes. The apical localization of alpha-DG on podocytes was more dominant than the basolateral localization. The basolateral staining with MoAb VIA4.1 was more pronounced than that of MoAb IIH6. With both MoAbs, the staining in rat kidneys was more prominent, in comparison to human kidneys. We conclude that alpha-DG is expressed at both the basolateral and apical sides of the podocyte. This localization suggests that alpha-DG plays a dual role in the maintenance of the unique architecture of podocytes by its binding to the glomerular basement membrane, and in the maintenance of the integrity of the filtration slit, respectively.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Distroglicanos / Podocitos Límite: Animals / Female / Humans Idioma: En Revista: J Histochem Cytochem Asunto de la revista: HISTOCITOQUIMICA Año: 2005 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Distroglicanos / Podocitos Límite: Animals / Female / Humans Idioma: En Revista: J Histochem Cytochem Asunto de la revista: HISTOCITOQUIMICA Año: 2005 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos