De novo formation of basal bodies in Naegleria gruberi: regulation by phosphorylation.
J Cell Biol
; 169(5): 719-24, 2005 Jun 06.
Article
en En
| MEDLINE
| ID: mdl-15939759
The de novo formation of basal bodies in Naegleria gruberi was preceded by the transient formation of a microtubule (MT)-nucleating complex containing gamma-tubulin, pericentrin, and myosin II complex (GPM complex). The MT-nucleating activity of GPM complexes was maximal just before the formation of visible basal bodies and then rapidly decreased. The regulation of MT-nucleating activity of GPM complexes was accomplished by a transient phosphorylation of the complex. Inhibition of dephosphorylation after the formation of basal bodies resulted in the formation of multiple flagella. 2D-gel electrophoresis and Western blotting showed a parallel relationship between the MT-nucleating activity of GPM complexes and the presence of hyperphosphorylated gamma-tubulin in the complexes. These data suggest that the nucleation of MTs by GPM complexes precedes the de novo formation of basal bodies and that the regulation of MT-nucleating activity of GPM complexes is essential to the regulation of basal body number.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Naegleria
/
Centro Organizador de los Microtúbulos
/
Flagelos
/
Microtúbulos
Límite:
Animals
Idioma:
En
Revista:
J Cell Biol
Año:
2005
Tipo del documento:
Article
Pais de publicación:
Estados Unidos