Nucleoplasmin-mediated unfolding of chromatin involves the displacement of linker-associated chromatin proteins.

Ramos, Isbaal; Prado, Adelina; Finn, Ron M; Muga, Arturo; Ausió, Juan

Ramos, Isbaal; Prado, Adelina; Finn, Ron M; Muga, Arturo; Ausió, Juan.

Afiliación

Ramos I; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada.

Biochemistry ; 44(23): 8274-81, 2005 Jun 14.

Article en En | MEDLINE | ID: mdl-15938617

RESUMEN

We have previously characterized the interaction of nucleoplasmin with core histones and studied the possible involvement of this chaperone molecule in transcription. Here we study the interaction of nucleoplasmin with chromatin. We show that highly phosphorylated Xenopus laevis egg nucleoplasmin can unfold sperm and somatic chromatin in a way that involves the removal of chromosomal proteins from linker DNA regions without a stable interaction with the nucleosome. The complexes between egg nucleoplasmin and both somatic and sperm-specific linker proteins have been hydrodynamically characterized using sedimentation equilibrium in the analytical ultracentrifuge. The results are discussed within the context of the possible implication of nucleoplasmin in processes such as transcription and replication licensing which take place after egg fertilization at the onset of development.

Asunto(s)

Cromatina/química; Proteínas Nucleares/química; Fosfoproteínas/química; Pliegue de Proteína; Proteínas de Xenopus/química; Animales; Pollos; Cromatina/metabolismo; Proteínas Cromosómicas no Histona/química; Proteínas Cromosómicas no Histona/metabolismo; Proteínas del Huevo/química; Proteínas del Huevo/metabolismo; Eritrocitos/química; Eritrocitos/metabolismo; Femenino; Histonas/química; Histonas/metabolismo; Masculino; Chaperonas Moleculares/química; Chaperonas Moleculares/metabolismo; Proteínas Nucleares/metabolismo; Nucleoplasminas; Nucleosomas/química; Nucleosomas/metabolismo; Oocitos/química; Oocitos/metabolismo; Fosfoproteínas/metabolismo; Proteínas de Xenopus/metabolismo; Xenopus laevis

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfoproteínas / Proteínas Nucleares / Cromatina / Pliegue de Proteína / Proteínas de Xenopus Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: Biochemistry Año: 2005 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos

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