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Single nucleotide polymorphisms in the gene encoding Krüppel-like factor 7 are associated with type 2 diabetes.
Kanazawa, A; Kawamura, Y; Sekine, A; Iida, A; Tsunoda, T; Kashiwagi, A; Tanaka, Y; Babazono, T; Matsuda, M; Kawai, K; Iiizumi, T; Fujioka, T; Imanishi, M; Kaku, K; Iwamoto, Y; Kawamori, R; Kikkawa, R; Nakamura, Y; Maeda, S.
Afiliación
  • Kanazawa A; Laboratory for Diabetic Nephropathy, SNP Research Centre, Institute of Physical and Chemical Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
Diabetologia ; 48(7): 1315-22, 2005 Jul.
Article en En | MEDLINE | ID: mdl-15937668
AIMS/HYPOTHESIS: Although genetic susceptibility plays an important role in the pathogenesis of type 2 diabetes, most of the genes that influence susceptibility to type 2 diabetes have yet to be identified. Krüppel-like transcription factors are known to play important roles in development and cell differentiation, and have recently been implicated in the pathogenesis of type 2 diabetes. The present study aimed to examine the associations of single nucleotide polymorphisms (SNPs) in genes encoding members of the Krüppel-like-factor (KLF) family with type 2 diabetes in a large cohort of Japanese subjects. METHODS: We genotyped 33 SNP loci found in 12 KLF genes in subjects with type 2 diabetes and in subjects from the general population using the PCR-Invader assay. We also examined the effects of the overexpression of KLF7 on adipogenesis in 3T3-L1 cells. RESULTS: We identified a significant association between an SNP in KLF7 and type 2 diabetes (A vs C: p=0.004 after Bonferroni's correction, odds ratio=1.59, 95% CI 1.27-2.00). The expression of Klf7 decreased in response to the differentiation of 3T3-L1 adipocytes, and the overexpression of KLF7 resulted in significant inhibition of adipogenesis in 3T3-L1 cells. CONCLUSIONS/INTERPRETATION: These results indicate that the gene encoding KLF7 is a novel candidate for conferring genetic susceptibility to type 2 diabetes.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Polimorfismo de Nucleótido Simple / Diabetes Mellitus Tipo 2 / Proteínas de Unión al ADN Tipo de estudio: Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Diabetologia Año: 2005 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Polimorfismo de Nucleótido Simple / Diabetes Mellitus Tipo 2 / Proteínas de Unión al ADN Tipo de estudio: Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Diabetologia Año: 2005 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania