fecB, a gene potentially involved in iron transport in Mycobacterium avium, is not induced within macrophages.
FEMS Microbiol Lett
; 247(2): 185-91, 2005 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-15935568
FecB is a protein involved in the transport of iron from ferric citrate in Escherichia coli and is present in the Mycobacterium tuberculosis genome sequence. Since the ability to retrieve iron from the host is crucial and may be related to virulence, we characterized the gene fecB from Mycobacterium avium, strain 101. An E. coli-mycobacterial shuttle plasmid with a fecB-promoter green fluorescence protein (gfp)-fusion was transformed into M. avium strain 104 to study the fecB-regulation. In vitro, the fecB expression in M. avium weakly correlated with the amount of iron present in the medium but the expression was maximal when there was no iron in the culture medium. In macrophages, M. avium fec B was not induced during the early phase of infection, suggesting that the iron concentration in the mycobacterial phagosome is not sufficiently low to stimulate the expression of fecB in M. avium.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Fagosomas
/
Complejo Mycobacterium avium
/
Regulación Bacteriana de la Expresión Génica
/
Bombas Iónicas
/
Macrófagos
Límite:
Animals
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
2005
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Reino Unido