[Primary study on the influence of blood transfusion on the STR genotypes of recipient's blood].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 36(3): 359-61, 414, 2005 May.
Article
en Zh
| MEDLINE
| ID: mdl-15931868
OBJECTIVE: To investigate whether any change occurred in recipients' blood collected at different times after transfusion with different quantity of blood. METHODS: Three patients were transfused with 400 ml, 800 ml and 1200 ml blood separately. The blood samples were collected from the recipients before transfusion and at 4 h, 8 h, 12 h after transfusion, and from the donors. DNA were extracted by Chelex-100 method and were amplified by the polymerase chain reaction (PCR). Six loci of D1S549, D18S865, D3S1754, D12S391, D12S375, D6S477 were selected. The PCR products were analyzed by polyacrylamide gel (PAG) vertical electrophoresis and silver staining. RESULTS: The investigations on the six STR loci revealed that the patients' STR genotypes remained unchanged within 12 h after blood transfusion. CONCLUSION: The STR genotypes of the donors would have no influence on the STR genotypes of the patients within 12 h after transfusion providing the volume of blood transfused is less than 1200 ml.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transfusión Sanguínea
/
Secuencias Repetidas en Tándem
/
Genotipo
Límite:
Humans
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Año:
2005
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China