Simultaneous quantification of six major active saponins of Panax notoginseng by high-performance liquid chromatography-UV method.
J Pharm Biomed Anal
; 38(1): 45-51, 2005 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-15907618
A simple, sensitive and specific high-performance liquid chromatography-UV (HPLC-UV) method has been developed for the first time to simultaneously quantify the six major active saponins of Panax notoginseng, namely notoginsenoside R1, ginsenoside Rg1, Rb1, Rg2, Rh1 and Rd. Astragaloside IV is used as the internal standard. This HPLC assay was performed on a reversed-phase C18 column with gradient elution of acetonitrile and 0.01% formic acid in 30 min. The method provided good reproducibility and sensitivity for the quantification of six saponins with overall intra- and inter-day precision and accuracy of less than 4.0% and higher than 90%, respectively. This assay is successfully applied to the determination of the six saponins in 23 notoginseng samples. The results indicated that the developed HPLC assay can be readily utilized as a quality control method for P. notoginseng.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saponinas
/
Espectrofotometría Ultravioleta
/
Cromatografía Líquida de Alta Presión
/
Panax
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2005
Tipo del documento:
Article
Pais de publicación:
Reino Unido