Alternative splicing facilitates internal ribosome entry on the ornithine decarboxylase mRNA.
Cell Mol Life Sci
; 62(11): 1267-74, 2005 Jun.
Article
en En
| MEDLINE
| ID: mdl-15905964
Ornithine decarboxylase (ODC) is the ratelimiting enzyme in the biosynthesis of polyamines, which are required for optimal cell growth and proliferation. ODC is overexpressed in many tumors and, conversely, its overexpression induces transformation. We have previously reported that ODC mRNA alternative splicing relieves the translation repression normally imposed by a long and structured 5' untranslated region (UTR), and that the ODC 5' UTR contains an internal ribosome entry site (IRES). Here we show that ODC IRES activity is enhanced following inclusion of alternative sequences generated by splicing at cryptic acceptor sites. Furthermore, the alternative ODC IRES is more sensitive to cell-cycledependent changes in the rate of translation. These findings uncover a new biological property of differentially spliced transcripts. This is the first example of alternative splicing that modulates mRNA translation through the cell cycle in a cap-independent manner.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ornitina Descarboxilasa
/
Ribosomas
/
ARN Mensajero
/
Empalme Alternativo
Límite:
Humans
Idioma:
En
Revista:
Cell Mol Life Sci
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2005
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Suiza