Spline-based image-to-volume registration for three-dimensional electron microscopy.
Ultramicroscopy
; 103(4): 303-17, 2005 Jul.
Article
en En
| MEDLINE
| ID: mdl-15885434
This paper presents an algorithm based on a continuous framework for a posteriori angular and translational assignment in three-dimensional electron microscopy (3DEM) of single particles. Our algorithm can be used advantageously to refine the assignment of standard quantized-parameter methods by registering the images to a reference 3D particle model. We achieve the registration by employing a gradient-based iterative minimization of a least-squares measure of dissimilarity between an image and a projection of the volume in the Fourier transform (FT) domain. We compute the FT of the projection using the central-slice theorem (CST). To compute the gradient accurately, we take advantage of a cubic B-spline model of the data in the frequency domain. To improve the robustness of the algorithm, we weight the cost function in the FT domain and apply a "mixed" strategy for the assignment based on the minimum value of the cost function at registration for several different initializations. We validate our algorithm in a fully controlled simulation environment. We show that the mixed strategy improves the assignment accuracy; on our data, the quality of the angular and translational assignment was better than 2 voxel (i.e., 6.54 angstroms). We also test the performance of our algorithm on real EM data. We conclude that our algorithm outperforms a standard projection-matching refinement in terms of both consistency of 3D reconstructions and speed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microscopía Electrónica
Idioma:
En
Revista:
Ultramicroscopy
Año:
2005
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Países Bajos