Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing.

Girish, Aparna; Sun, Hongyan; Yeo, Dawn S Y; Chen, Grace Y J; Chua, Teck-Khiang; Yao, Shao Q

Girish, Aparna; Sun, Hongyan; Yeo, Dawn S Y; Chen, Grace Y J; Chua, Teck-Khiang; Yao, Shao Q.

Afiliación

Girish A; Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

Bioorg Med Chem Lett ; 15(10): 2447-51, 2005 May 16.

Article en En | MEDLINE | ID: mdl-15863295

RESUMEN

One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system.

Asunto(s)

Inteínas; Análisis por Matrices de Proteínas; Proteínas/química; Electroforesis en Gel de Poliacrilamida

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Análisis por Matrices de Proteínas / Inteínas Idioma: En Revista: Bioorg Med Chem Lett Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2005 Tipo del documento: Article País de afiliación: Singapur Pais de publicación: Reino Unido

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