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A new variant t(6;15;17)(q25;q22;q21) in acute promyelocytic leukemia: fluorescence in situ hybridization confirmation.
Eclache, Virginie; Viguie, Franck; Frocrain, Claudie; Cassinat, Bruno; Chomienne, Christine; Cymbalista, Florence; Fenaux, Pierre.
Afiliación
  • Eclache V; Laboratoire d'Hématologie, Hôpital Jean Verdier, Bondy, France. eclache@avc.ap-hop-paris.fr
Cancer Genet Cytogenet ; 159(1): 69-73, 2005 May.
Article en En | MEDLINE | ID: mdl-15860361
Acute promyelocytic leukemia (APL) is characterized by the t(15;17)(q22;q21), which results in the fusion of the promyelocytic leukemia (PML) gene at 15q22 with the retinoic acid alpha-receptor (RARalpha) at 17q21. The 2 chimeric genes PML/RARalpha and RARalpha/PML are thought to play a role in leukemogenesis. We report a case of APL in a patient carrying an apparently complex variant translocation identified as t(6;15;17) by R-banding and whole chromosome 15 and 17 painting. However, FISH analysis with a PML/RARalpha dual-color kit showed a more complex translocation, resulting presumably from a two-step rearrangement, with PML-RARalpha fusion gene located as expected on the der(15) but the residual 5'-RARalpha signal located on the der(6). The patient achieved complete remission with all-trans retinoic acid treatment associated with chemotherapy. This case illustrates the usefulness of combined cytogenetics, FISH, and molecular biology to evidence the PML/RARalpha fusion gene in complex cases.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Translocación Genética / Cromosomas Humanos Par 6 / Cromosomas Humanos Par 15 / Cromosomas Humanos Par 17 / Leucemia Promielocítica Aguda Tipo de estudio: Prognostic_studies Límite: Female / Humans / Middle aged Idioma: En Revista: Cancer Genet Cytogenet Año: 2005 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Translocación Genética / Cromosomas Humanos Par 6 / Cromosomas Humanos Par 15 / Cromosomas Humanos Par 17 / Leucemia Promielocítica Aguda Tipo de estudio: Prognostic_studies Límite: Female / Humans / Middle aged Idioma: En Revista: Cancer Genet Cytogenet Año: 2005 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos