Conservation of biological properties of the CD40 ligand, CD154 in a non-mammalian vertebrate.
Dev Comp Immunol
; 29(4): 361-74, 2005.
Article
en En
| MEDLINE
| ID: mdl-15859239
Signals delivered by the CD40 ligand, CD154, have crucial roles in immune responses in mammals, being required for development of germinal centres, maturation of T-dependent antibody responses, and generation of B-cell memory. To determine whether these functions were conserved in a non-mammalian species, a putative chicken CD 154 cDNA was used to make an oligomeric fusion protein, and to raise monoclonal antibodies. The antibodies detected surface expression on activated T-cells. The fusion protein detected expression of a receptor on B-cells, thrombocytes and macrophages. Biological effects of the fusion protein included induction of NO synthesis in a macrophage cell line, enhancement of splenic B-cell survival, and induction of apoptosis in a bursal lymphoma cell line. These observations demonstrated substantial functional equivalence with mammalian CD 154 and thus provided evidence for the early evolutionary emergence of the set of functions associated with this molecule, and its central role in the vertebrate immune system.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pollos
/
Evolución Molecular
/
Ligando de CD40
Límite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Año:
2005
Tipo del documento:
Article
Pais de publicación:
Estados Unidos