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Mild hypothermia protects against sodium taurocholate (NaTc)-induced acute pancreatitis in rats with adverse effects on serum cytokines.
Wang, XueMin; Jiang, Wei; Zhao, Gang; Du, DongPing; Zhou, Ming; Hang, YanNan; Tong, Chuanyao.
Afiliación
  • Wang X; Department of Intensive Care Unit, Shanghai 6th Municipal Hospital, Medical College of Shanghai Jiao Tong University, Shanghai, China. wangxm@sjtu.edu.cn
Pancreas ; 30(4): e80-6, 2005 May.
Article en En | MEDLINE | ID: mdl-15841032
OBJECTIVES: To test the effect of mild hypothermia in reducing pancreatic damage and improving the survival of acute hemorrhagic and necrotic pancreatitis (AHNP) in rats. METHODS: Thirty-six male Sprague-Dawley rats were studied. Sham animals (n = 12) received laparotomy and normothermia; for AHNP with normothermia (n = 12), the core temperature was maintained at 37 degrees C to 37.5 degrees C after AHNP for 2 to 5 hours; for AHNP with hypothermia (n = 12), the core temperature was maintained at 32 degrees C to 33 degrees C afterward. Serum amylase and lipase, interleukin (IL)-1beta, and tumor necrosis factor (TNF-alpha) were measured. The pancreas was examined histologically. In a separate experiment, the core temperature was kept either in normothermia (n = 20) or mild hypothermia (n = 20) for 12 hours after the induction of AHNP; the animals were then returned to their cage. The survival rate was monitored up to 72 hours. RESULTS: Mild hypothermia significantly decreased the release of serum amylase and lipase compared with AHNP normothermia at 2 and 5 hours. Histology examination revealed significantly less tissue damage in AHNP hypothermia. There was a further increase in TNF-alpha and IL-1beta in AHNP in animals with hypothermia compared with normothermia animals. The application of mild hypothermia significantly improved the survival in animals induced with AHNP. CONCLUSIONS: Mild hypothermia decreases amylase and lipase release and improves a survival of AHNP in rats.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interleucina-1 / Factor de Necrosis Tumoral alfa / Pancreatitis Aguda Necrotizante / Hipotermia Inducida Límite: Animals Idioma: En Revista: Pancreas Asunto de la revista: GASTROENTEROLOGIA Año: 2005 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interleucina-1 / Factor de Necrosis Tumoral alfa / Pancreatitis Aguda Necrotizante / Hipotermia Inducida Límite: Animals Idioma: En Revista: Pancreas Asunto de la revista: GASTROENTEROLOGIA Año: 2005 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos