Efficient formation of bipolar microtubule bundles requires microtubule-bound gamma-tubulin complexes.
J Cell Biol
; 169(2): 297-308, 2005 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-15837798
The mechanism for forming linear microtubule (MT) arrays in cells such as neurons, polarized epithelial cells, and myotubes is not well understood. A simpler bipolar linear array is the fission yeast interphase MT bundle, which in its basic form contains two MTs that are bundled at their minus ends. Here, we characterize mto2p as a novel fission yeast protein required for MT nucleation from noncentrosomal gamma-tubulin complexes (gamma-TuCs). In interphase mto2Delta cells, MT nucleation was strongly inhibited, and MT bundling occurred infrequently and only when two MTs met by chance in the cytoplasm. In wild-type 2, we observed MT nucleation from gamma-TuCs bound along the length of existing MTs. We propose a model on how these nucleation events can more efficiently drive the formation of bipolar MT bundles in interphase. Key to the model is our observation of selective antiparallel binding of MTs, which can both explain the generation and spatial separation of multiple bipolar bundles.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Schizosaccharomyces
/
Tubulina (Proteína)
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División Celular
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Proteínas de Schizosaccharomyces pombe
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Proteínas Asociadas a Microtúbulos
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Microtúbulos
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Huso Acromático
Idioma:
En
Revista:
J Cell Biol
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos