Co-transfection of messenger RNA and siRNA as a method to study the efficiency of siRNA.

Mutzke, Thorsten; Schubkegel, Gudrun; Teufel, Regina; Ketterer, Thomas; Probst, Jochen; Scheel, Birgit; Carralot, Jean-Philippe; Pascolo, Steve; Ghoreschi, Kamran; Weigert, Christina

Mutzke, Thorsten; Schubkegel, Gudrun; Teufel, Regina; Ketterer, Thomas; Probst, Jochen; Scheel, Birgit; Carralot, Jean-Philippe; Pascolo, Steve; Ghoreschi, Kamran; Weigert, Christina.

Afiliación

Mutzke T; CureVac GmbH, Tübingen, Germany.

Nucleosides Nucleotides Nucleic Acids ; 24(2): 147-52, 2005.

Article en En | MEDLINE | ID: mdl-15822620

RESUMEN

The definition of an optimal siRNA results from the in vitro testing of several siRNA designed to specifically target a gene. Usually, such in vitro tests consist in the transfection of the several siRNA duplexes in a cell expressing stably the gene of interest. When a siRNA specific for a mRNA coding toxic proteins (certain transcription factors, transporters, toxins, cell cycle controlling proteins, etc.) must be tested, the generation of a target cell is difficult. Here we report a quick method to test the efficiency of a siRNA through its co-transfection with the targeted mRNA. This technique can be used as a fast method to test siRNA even when they target genes that cannot be stably expressed in the cells of interest.

Asunto(s)

Interferencia de ARN; ARN Mensajero/genética; ARN Interferente Pequeño/genética; Animales; Células Cultivadas; Células Dendríticas; Electroporación; Expresión Génica; Ratones; Transfección

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Mensajero / ARN Interferente Pequeño / Interferencia de ARN Límite: Animals Idioma: En Revista: Nucleosides Nucleotides Nucleic Acids Asunto de la revista: BIOQUIMICA Año: 2005 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

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