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Adaptation of Spirogyra insignis (Chlorophyta) to an extreme natural environment (sulphureous waters) through preselective mutations.
Flores-Moya, Antonio; Costas, Eduardo; Bañares-España, Elena; García-Villada, Libertad; Altamirano, María; López-Rodas, Victoria.
Afiliación
  • Flores-Moya A; Departamento de Biología Vegetal (Botánica), Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, Spain. floresa@uma.es
New Phytol ; 166(2): 655-61, 2005 May.
Article en En | MEDLINE | ID: mdl-15819927
Adaptation of Spirogyra insignis (Chlorophyceae) to growth and survival in an extreme natural environment (sulphureous waters from La Hedionda Spa, S. Spain) was analysed by using an experimental model. Photosynthesis and growth of the alga were inhibited when it was cultured in La Hedionda Spa waters (LHW), but after further incubation for several weeks, the culture survived due to the growth of a variant that was resistant to LHW. A Luria-Delbruck fluctuation analysis was carried out to distinguish between resistant filaments arising from rare spontaneous mutations and resistant filaments arising from other mechanisms of adaptation. It was demonstrated that the resistant filaments arose randomly by rare spontaneous mutations before the addition of LHW (preselective mutations). The rate of spontaneous mutation from sensitivity to resistance was 2.7 x 10(-7) mutants per cell division. Since LHW(resistant) mutants have a diminished growth rate, they are maintained in nonsulphureous natural waters as the result of a balance between new resistants arising from spontaneous mutation and resistants eliminated by natural selection. Thus, recurrence of rare spontaneous preselective mutations ensures the survival of the alga in sulphureous waters.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Azufre / Agua / Ecosistema / Chlorophyta Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2005 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Azufre / Agua / Ecosistema / Chlorophyta Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2005 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido