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c-src activating mutation analysis in Chinese patients with colorectal cancer.
Tan, Ye-Xiong; Wang, Han-Tao; Zhang, Peng; Yan, Zhong-Hua; Dai, Guan-Long; Wu, Meng-Chao; Wang, Hong-Yang.
Afiliación
  • Tan YX; International Co-operational Laboratory on Signal Transduction, Eastern Hepatobiliary Institute, Shanghai 200438, China.
World J Gastroenterol ; 11(15): 2351-3, 2005 Apr 21.
Article en En | MEDLINE | ID: mdl-15818752
AIM: To investigate the occurrence of cellular src (c-src) activating mutation at codon 531 in colorectal cancer patients from Chinese mainland. METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay followed by sequencing and single-strand conformation polymor-phism analysis were carried out to screen 110 samples of primary colorectal cancer and 20 colorectal liver metastases. RESULTS: Only one sample showed PCR-RFLP-positive results and carried somatic codon 531 mutations. No additional mutation of c-src exon 12 was found. CONCLUSION: c-src codon 531 mutation in colorectal cancer is not the cause of c-src activation.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Genes src / Pueblo Asiatico Límite: Adult / Aged / Humans / Middle aged Idioma: En Revista: World J Gastroenterol Asunto de la revista: GASTROENTEROLOGIA Año: 2005 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Genes src / Pueblo Asiatico Límite: Adult / Aged / Humans / Middle aged Idioma: En Revista: World J Gastroenterol Asunto de la revista: GASTROENTEROLOGIA Año: 2005 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos