Characterization of a cyclooxygenase-2-765G-->C promoter polymorphism in human neural cells.
Neuroreport
; 16(6): 575-9, 2005 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-15812311
Direct sequencing of the human cyclooxygenase-2 gene promoter revealed a common single nucleotide substitution, cyclooxygenase-2-765G-->C, in 24.5% of the populations analyzed. This change introduced a 20 base pair polypyrimidine/polypurine element and a partial recognition feature for RXRalpha, the 9-cis retinoic acid receptor, into the polymorphic promoter. Cyclooxygenase-2-765G-->C constructs, when transfected into human neural cells, exhibited a 1.4-fold higher level of basal expression, while the proinflammatory factors interleukin-1beta and 9-cis retinoic acid synergistically induced polymorphic promoter activity 2.4-fold over wild type. These results suggest that under specific conditions of cellular stress, a common variation in cyclooxygenase-2 promoter structure may enhance cyclooxygenase-2 transcription, and this may contribute to the proliferation of an inflammatory response in brain cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Encefalopatías
/
Prostaglandina-Endoperóxido Sintasas
/
Polimorfismo de Nucleótido Simple
/
Neuronas
Límite:
Humans
Idioma:
En
Revista:
Neuroreport
Asunto de la revista:
NEUROLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Reino Unido