Insulinlike growth factor I gene expression is increased in the fetal lung after tracheal ligation.

Frenckner, Björn; Eklöf, Ann-Christine; Eriksson, Håkan; Masironi, Britt; Sahlin, Lena

Frenckner, Björn; Eklöf, Ann-Christine; Eriksson, Håkan; Masironi, Britt; Sahlin, Lena.

Afiliación

Frenckner B; Division of Pediatric Surgery, Astrid Lindgren Children's Hospital, Q3:03, SE-171 76, Sweden. bjorn.frenckner@karolinska.se

J Pediatr Surg ; 40(3): 457-63, 2005 Mar.

Article en En | MEDLINE | ID: mdl-15793718

RESUMEN

BACKGROUND/PURPOSE: The mortality and morbidity in congenital diaphragmatic hernia are mainly caused by pulmonary hypoplasia. To improve clinical results, further methods inducing lung growth may have to be used. The aim of this report was to evaluate the expression of insulinlike growth factor I (IGF-I), estrogen receptor alpha, estrogen receptor beta, growth hormone receptor, and thioredoxin in a rat model of hypoplastic, hyperplastic, and normal fetal lungs to improve understanding of lung growth. METHODS: Hypoplastic diaphragmatic hernia lungs were created by giving nitrofen by gavage to pregnant rats on day 9.5. Hyperplastic lungs were achieved by intrauterine tracheal ligation of rat fetuses on day 19. All lungs were harvested on gestational day 21. Total nucleic acids were extracted by proteinase K digestion and extraction in phenol/chloroform. The total nucleic acids mixture was hybridized with radioactively labeled RNA probes, and the radioactivity of the hybrids was compared with the respective standard curve of known amounts of in vitro synthesized mRNA. Immunohistochemistry staining was performed for IGF-I. RESULTS: The IGF-I mRNA was significantly (P < .01) higher in hyperplastic lungs compared with control and hypoplastic lungs. The latter 2 did not differ. No difference was found between the other mRNA levels in the study groups. CONCLUSIONS: IGF-I is involved in the accelerated lung growth seen after intrauterine tracheal ligation.

Asunto(s)

Proteínas Fetales/biosíntesis; Regulación del Desarrollo de la Expresión Génica; Hernia Diafragmática/embriología; Factor I del Crecimiento Similar a la Insulina/biosíntesis; Pulmón/embriología; Tráquea/embriología; Anomalías Inducidas por Medicamentos/genética; Anomalías Inducidas por Medicamentos/metabolismo; Anomalías Inducidas por Medicamentos/patología; Animales; Modelos Animales de Enfermedad; Receptor alfa de Estrógeno/biosíntesis; Receptor alfa de Estrógeno/genética; Receptor beta de Estrógeno/biosíntesis; Receptor beta de Estrógeno/genética; Femenino; Proteínas Fetales/genética; Edad Gestacional; Hernia Diafragmática/genética; Hernia Diafragmática/metabolismo; Hiperplasia; Factor I del Crecimiento Similar a la Insulina/genética; Ligadura; Pulmón/anomalías; Pulmón/metabolismo; Pulmón/patología; Éteres Fenílicos/toxicidad; Embarazo; ARN Mensajero/biosíntesis; ARN Mensajero/genética; Ratas; Ratas Sprague-Dawley; Receptores de Somatotropina/biosíntesis; Receptores de Somatotropina/genética; Tiorredoxinas/biosíntesis; Tiorredoxinas/genética; Tráquea/cirugía

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tráquea / Factor I del Crecimiento Similar a la Insulina / Regulación del Desarrollo de la Expresión Génica / Proteínas Fetales / Hernia Diafragmática / Pulmón Tipo de estudio: Prognostic_studies Idioma: En Revista: J Pediatr Surg Año: 2005 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos

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