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'Rescue' of mini-genomic constructs and viruses by combinations of morbillivirus N, P and L proteins.
Brown, D D; Collins, F M; Duprex, W P; Baron, M D; Barrett, T; Rima, B K.
Afiliación
  • Brown DD; School of Biology and Biochemistry, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.
  • Collins FM; School of Biology and Biochemistry, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.
  • Duprex WP; Centre for Cancer Research and Cell Biology, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.
  • Baron MD; School of Biology and Biochemistry, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.
  • Barrett T; The Institute for Animal Health, Pirbright, Surrey, UK.
  • Rima BK; The Institute for Animal Health, Pirbright, Surrey, UK.
J Gen Virol ; 86(Pt 4): 1077-1081, 2005 Apr.
Article en En | MEDLINE | ID: mdl-15784901
Chloramphenicol acetyltransferase (CAT)-expressing negative-sense mini-genomic constructs of measles virus (MV) and rinderpest virus (RPV) were rescued by standard technology with helper plasmids expressing the nucleocapsid (N), phospho- (P) and large (L) proteins of MV, canine distemper virus (CDV) or RPV in order to determine whether the proteins of different viruses can function together. Homogeneous sets consisting of N, P and L plasmids derived from one virus were able to generate reporter gene expression from either mini-genomic construct. Heterogeneous sets of proteins from different viruses were not functional, with the exception that a low level of activity was obtained when MV N and P protein were combined with RPV L protein in the rescue of the MV mini-genomic construct, or CDV N was combined with RPV P and L in the rescue of the RPV mini-genome. However, only homogeneous sets of plasmids were able to rescue infectious virus from full-length anti-genome-expressing plasmids.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plásmidos / Recombinación Genética / Virus de la Peste Bovina / Proteínas Virales / Genoma Viral / Virus del Sarampión Límite: Animals / Humans Idioma: En Revista: J Gen Virol Año: 2005 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plásmidos / Recombinación Genética / Virus de la Peste Bovina / Proteínas Virales / Genoma Viral / Virus del Sarampión Límite: Animals / Humans Idioma: En Revista: J Gen Virol Año: 2005 Tipo del documento: Article Pais de publicación: Reino Unido