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Binding of human papillomavirus 16 E6 to p53 and E6AP is impaired by monoclonal antibodies directed against the second zinc-binding domain of E6.
Lagrange, Magali; Charbonnier, Sebastian; Orfanoudakis, Georges; Robinson, Philip; Zanier, Katia; Masson, Murielle; Lutz, Yves; Trave, Gilles; Weiss, Etienne; Deryckere, François.
Afiliación
  • Lagrange M; UMR7100, Ecole Supérieure de Biotechnologie de Strasbourg, Pole API, boulevard Sébastien Brant, 67412 Illkirch Cedex, France.
  • Charbonnier S; UMR7100, Ecole Supérieure de Biotechnologie de Strasbourg, Pole API, boulevard Sébastien Brant, 67412 Illkirch Cedex, France.
  • Orfanoudakis G; UMR7100, Ecole Supérieure de Biotechnologie de Strasbourg, Pole API, boulevard Sébastien Brant, 67412 Illkirch Cedex, France.
  • Robinson P; UMR7100, Ecole Supérieure de Biotechnologie de Strasbourg, Pole API, boulevard Sébastien Brant, 67412 Illkirch Cedex, France.
  • Zanier K; UMR7100, Ecole Supérieure de Biotechnologie de Strasbourg, Pole API, boulevard Sébastien Brant, 67412 Illkirch Cedex, France.
  • Masson M; UMR7100, Ecole Supérieure de Biotechnologie de Strasbourg, Pole API, boulevard Sébastien Brant, 67412 Illkirch Cedex, France.
  • Lutz Y; IGBMC, CNRS/INSERM/ULP, 1 rue Laurent Fries, BP 10142, 67404 Illkirch Cedex, France.
  • Trave G; UMR7100, Ecole Supérieure de Biotechnologie de Strasbourg, Pole API, boulevard Sébastien Brant, 67412 Illkirch Cedex, France.
  • Weiss E; UMR7100, Ecole Supérieure de Biotechnologie de Strasbourg, Pole API, boulevard Sébastien Brant, 67412 Illkirch Cedex, France.
  • Deryckere F; UMR7100, Ecole Supérieure de Biotechnologie de Strasbourg, Pole API, boulevard Sébastien Brant, 67412 Illkirch Cedex, France.
J Gen Virol ; 86(Pt 4): 1001-1007, 2005 Apr.
Article en En | MEDLINE | ID: mdl-15784893
The E6 protein of cancer-associated human papillomavirus type 16 (16E6) binds to p53 and, in association with E6AP, promotes its degradation through the ubiquitin-proteasome pathway. The aim of this work was to develop monoclonal antibodies against 16E6 and to test their effect on the binding of 16E6 to p53 and E6AP, and on the degradation of p53. It was shown that an antibody directed against the N terminus of 16E6 inhibited E6AP-dependent binding to p53 and degradation of p53, whereas two different antibodies directed to the second zinc-binding domain of 16E6 reduced 16E6 E6AP-independent binding to p53 and binding to E6AP but not degradation of p53.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papillomaviridae / Proteínas Represoras / Proteínas Oncogénicas Virales / Proteína p53 Supresora de Tumor / Ubiquitina-Proteína Ligasas / Anticuerpos Monoclonales Límite: Animals / Humans Idioma: En Revista: J Gen Virol Año: 2005 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papillomaviridae / Proteínas Represoras / Proteínas Oncogénicas Virales / Proteína p53 Supresora de Tumor / Ubiquitina-Proteína Ligasas / Anticuerpos Monoclonales Límite: Animals / Humans Idioma: En Revista: J Gen Virol Año: 2005 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido