Effect of a booster dose of serogroup B meningococcal vaccine on antibody response to Neisseria meningitidis in mice vaccinated with different immunization schedules.
FEMS Immunol Med Microbiol
; 44(1): 35-42, 2005 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-15780576
The generation and maintenance of memory antibody response by different primary immunization schedules with the Cuban-produced outer membrane protein based vaccine was investigated in a murine model. We analyzed the duration of the antibody response (IgG-ELISA and bactericidal titer) and the effect of a booster dose on the antibody response. The IgG avidity index was determined in an attempt to find a marker for memory development. This study also included an analysis of IgG subclasses induced by primary and booster immunization. The specificity of bactericidal antibodies was investigated using local strains of the same serotype/serosubtype (4,7:P1.19,15) as the vaccine strain and mutant strains lacking major outer membrane proteins. A significant recall response was induced by a booster dose given 7 months after a primary series of 2, 3 or 4 doses of vaccine. The primary antibody response showed a positive dose-effect. In contrast, a negative dose-effect was found on the booster bactericidal antibody response. There was a significant increase in IgG1 levels after the fourth and booster doses. Three doses of vaccine were required to induce a significant increase in IgG avidity. Two injections of vaccine induced a significant antibody response to PorA protein, while 4 injections induced a larger range of specificities.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vacunas Meningococicas
/
Anticuerpos Antibacterianos
/
Neisseria meningitidis
Límite:
Animals
Idioma:
En
Revista:
FEMS Immunol Med Microbiol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Reino Unido