[The effects of culture supernatant of bladder tumor cells on formation and function of dendritic cells].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 21(2): 237-9, 2005 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-15766415
AIM: To explore the effects of culture supernatant of bladder tumor cell lines BIU-87 on the development and function of peripheral blood mononuclear cell (PBMC)-derived dendritic cells. METHODS: The culture supernatant of BIU-87 cells was collected and used as the conditional culture medium for PBMC-derived DCs culture in experiment group. Normal cultured DCs were used as control group. The phenotypes of DCs in experiment group and in control group were analyzed by FACS. The capacity and difference to stimulate allogenetic T cells of PBMC-derived DCs from the two groups were evaluated by MTT colourimetry. RESULTS: Phenotypic analysis showed that DCs co-cultured with culture supernatant of BIU-87 cells expressed lower levels of CD1a, CD83 and CD86. Moreover, as compared with control group, the maturation of DCs was inhibited, and the capacity to stimulate allgentic T cell proliferation by DCs descended(P<0.05). CONCLUSION: The culture supernatant of BIU-87 cells can inhibit the development and function of DCs. It is a possible reason for the decrease of the number of DCs and the descent of its functional in the patients with bladder tumor.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Vejiga Urinaria
/
Células Dendríticas
/
Medios de Cultivo Condicionados
Límite:
Humans
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China