Norepinephrine transporter immunoblotting and radioligand binding in cocaine abusers.
J Neurosci Methods
; 143(1): 79-85, 2005 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-15763139
The norepinephrine transporter (NET) is a membrane protein responsible for transporting extracellular norepinephrine. The cocaine and tricyclic antidepressant-sensitive NET belongs to a family of sodium and chloride coupled transporters that include the monoamines dopamine and serotonin and the amino acids GABA and glycine. The regional distribution of the NET has been defined by synaptosomal uptake of norepinephrine and by autoradiographic approaches in rodent and primate brain. However, the NET has not been well characterized in the human brain due to the overall low abundance of protein expressed in axon terminals. Recently, immunolocalization studies have been used to identify the regional distribution of the cytoplasmic NET epitope in rodent brain. We report here on the characteristics of drug interactions with the native NET protein in human postmortem brain. Antisera raised against a 17-amino acid peptide from the N-terminus of the hNET recognized an 80 kDa species in human cerebral cortex. Chronic exposure to cocaine upregulated NET protein expression and [3H]nisoxetine binding sites in the insular cortex from brains of cocaine addicts. These results demonstrate that immunologic and radioligand binding approaches afford specific labeling of the native transport protein in postmortem human brain.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ensayo de Unión Radioligante
/
Immunoblotting
/
Fluoxetina
/
Cocaína
/
Trastornos Relacionados con Cocaína
/
Simportadores
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Neurosci Methods
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Países Bajos