Single step purification of a series of wheat recombinant proteins with expanded bed absorption chromatography.

de Lamotte, F

de Lamotte, F.

Afiliación

de Lamotte F; UMR Polymorphismes d'Intérêts Agronomiques-1096, INRA/CIRAD/ENSA-M, 2 Place Viala, 34060 Montpellier, Cedex 01, France. lamotte@ensam.inra.fr

J Chromatogr B Analyt Technol Biomed Life Sci ; 818(1): 29-33, 2005 Apr 15.

Article en En | MEDLINE | ID: mdl-15722041

RESUMEN

Expanded bed absorption chromatography (EBA) was used to improve and simplify the purification of several wheat recombinant proteins. Binding and elution conditions were set to allow the purification of the over expressed protein in a single step. In comparison with our previous multi step protocol, same purity was obtained while EBA required less time (one day instead of five) and gave a higher yield (63% instead of 10%). This new procedure was then used for the successful purification of five other wheat ns-LTP. Despite their important polymorphism (identity from 44 to 97 %-pHi from 8 to 10), the EBA protocol allowed their purification in a single step.

Asunto(s)

Cromatografía de Afinidad/métodos; Proteínas de Plantas/aislamiento & purificación; Proteínas Recombinantes/aislamiento & purificación; Triticum/metabolismo; Adsorción; Proteínas de la Membrana/aislamiento & purificación; Proteínas de Transferencia de Fosfolípidos/aislamiento & purificación; Pichia/metabolismo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Triticum / Proteínas Recombinantes / Cromatografía de Afinidad Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2005 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos

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