Tomographic fluorescence imaging in tissue phantoms: a novel reconstruction algorithm and imaging geometry.
IEEE Trans Med Imaging
; 24(2): 137-54, 2005 Feb.
Article
en En
| MEDLINE
| ID: mdl-15707240
A novel image reconstruction algorithm has been developed and demonstrated for fluorescence-enhanced frequency-domain photon migration (FDPM) tomography from measurements of area illumination with modulated excitation light and area collection of emitted fluorescence light using a gain modulated image-intensified charge-coupled device (ICCD) camera. The image reconstruction problem was formulated as a nonlinear least-squares-type simple bounds constrained optimization problem based upon the penalty/modified barrier function (PMBF) method and the coupled diffusion equations. The simple bounds constraints are included in the objective function of the PMBF method and the gradient-based truncated Newton method with trust region is used to minimize the function for the large-scale problem (39919 unknowns, 2973 measurements). Three-dimensional (3-D) images of fluorescence absorption coefficients were reconstructed using the algorithm from experimental reflectance measurements under conditions of perfect and imperfect distribution of fluorophore within a single target. To our knowledge, this is the first time that targets have been reconstructed in three-dimensions from reflectance measurements with a clinically relevant phantom.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrofotometría Infrarroja
/
Algoritmos
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Interpretación de Imagen Asistida por Computador
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Aumento de la Imagen
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Tejido Conectivo
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Tomografía Óptica
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Microscopía Fluorescente
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
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Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
IEEE Trans Med Imaging
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos