Immunochemical analysis of lipopolysaccharide-like component extracted from Borrelia burgdorferi sensu lato.
Folia Microbiol (Praha)
; 49(5): 625-9, 2004.
Article
en En
| MEDLINE
| ID: mdl-15702557
Immunoelectrophoresis and its modifications were applied to analysis of a lipopolysaccharide-like component (LPS-LC) extracted from Borrelia garinii strains K24 and K48 isolated from Ixodes ricinus and Borrelia burgdorferi sensu stricto strain B31. A modification of the hot phenol-water method was used for isolation of LPS. Immunoelectrophoresis (IE) and crossed immunoelectrophoresis (CIE) of LPS-LC with polyclonal rabbit antisera revealed a pattern and properties partially similar to LPS from other Gram-negative bacteria. B. garinii LPS-LC formed in CIE a diffuse band extending from the start to the anode. Similarly, the shape and position of the band in IE did not show major differences from LPS of other Gram-negative bacteria. The LPS-LC extracted from the three genomic groups of B. burgdorferi sensu lato were found to have similar immunochemical properties irrespective of their genotype origin.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Grupo Borrelia Burgdorferi
/
Lipopolisacáridos
Límite:
Animals
Idioma:
En
Revista:
Folia Microbiol (Praha)
Año:
2004
Tipo del documento:
Article
País de afiliación:
Eslovaquia
Pais de publicación:
Estados Unidos