N-oxide sensing in Pseudomonas aeruginosa: expression and preliminary characterization of DNR, an FNR-CRP type transcriptional regulator.
Biochem Soc Trans
; 33(Pt 1): 184-6, 2005 Feb.
Article
en En
| MEDLINE
| ID: mdl-15667301
In denitrifying bacteria, the concentration of NO is maintained low by a tight control of the expression and activity of nitrite and NO reductases. Regulation involves redox-linked transcription factors, such as those belonging to the CRP-FNR (cAMP receptor protein-fumarate and nitrate reductase regulator) superfamily, which act as oxygen and N-oxide sensors. Given that few members of this superfamily have been characterized in detail, we have cloned, expressed and purified the dissimilative nitrate respiration regulator from Pseudomonas aeruginosa. To gain insights on the structural properties of the dissimilative nitrate respiration regulator, we have also determined the aggregation state of the purified protein and its ability to bind hydrophobic compounds such as 8-anilino-1-naphthalenesulphonic acid.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Óxidos
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Pseudomonas aeruginosa
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Proteínas Bacterianas
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Factores de Transcripción
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Transcripción Genética
Idioma:
En
Revista:
Biochem Soc Trans
Año:
2005
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Reino Unido