A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce ATP and cyclic AMP which appear to be secretory mediators.
Microbiol Immunol
; 49(1): 73-8, 2005.
Article
en En
| MEDLINE
| ID: mdl-15665456
The hemolysin of Vibrio mimicus(VMH) is a pore-forming toxin with both enterotoxic and hemolytic activity. The hemolysis by VMH is induced by creation of pores in the membrane of erythrocyte; however, the mechanism for the enterotoxic action of VMH has remained unclear. In order to clarify the mechanism, we incubated T84 cells (a human colon carcinoma cell line) with VMH and found that the levels of ATP and cyclic AMP of culture medium increased after exposure of the cells to VMH. Subsequently, we found that the fluid accumulating activity of VMH in a mouse internal loop assay was reduced by administration of glibenclamide, an inhibitor of cyclic AMP-dependent chloride channels, into the intestinal loop. These results suggest that the stimulation of cells to produce nucleotides by VMH is linked to the enterotoxic activity of the toxin.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Adenosina Trifosfato
/
AMP Cíclico
/
Vibrio mimicus
/
Enterotoxinas
/
Proteínas Hemolisinas
Límite:
Humans
Idioma:
En
Revista:
Microbiol Immunol
Año:
2005
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Australia