[ATHK1 gene regulates signal transduction of osmotic stress in Arabidopsis thaliana].
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
; 30(5): 553-60, 2004 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-15627710
Differences in physiology and gene expression between ATHK1 knock-out mutant caused by T-DNA insertion and wild type (WT) of WS accession of Arabidopsis thaliana were analysed. Water loss ratio of detached leaf of ATHK1-mutant was obviously higher than that of WT. After being treated with 30% PEG-6000, ion leakage ratio of cell membrane in wild type leaves was 50% higher than that before PEG treatment, while in mutant leaves it increased 80%. The wilted phenotype of ATHK1-mutant after PEG treatment for 48 h was higher than that of WT. All these results showed that ATHK1-mutant was more sensitive to osmotic stress compared to WT and ATHK1 involved in osmotic stress adaptation. Differential-Display Reverse Transcription-PCR (DDRT-PCR) analysis was carried out to investigate the difference of gene expression between ATHK1-mutant and WT. Nine differential cDNA fragments involved in stress adaptation were identified, including the MAPKKK18 and serine/threonine protein kinase genes. These fragments were up-regulated by PEG treatment in WT, but not in ATHK1-mutant. These results indicate that ATHK1 plays an important role up-stream from MAPK in the osmotic stress signal transduction pathway. ATHK1 may be working as a plant osmosensor.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transducción de Señal
/
Arabidopsis
/
Proteínas de Transporte de Catión
/
Simportadores
/
Proteínas de Arabidopsis
Idioma:
Zh
Revista:
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Año:
2004
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China