Squirrel monkeys support replication of BK virus more efficiently than simian virus 40: an animal model for human BK virus infection.
J Virol
; 79(2): 1320-6, 2005 Jan.
Article
en En
| MEDLINE
| ID: mdl-15613359
We performed experiments to test the suitability of squirrel monkeys (Saimiri sciureus) as an experimental model for BK virus (BKV) and simian virus 40 (SV40) infection. Four squirrel monkeys received intravenous inoculation with BKV Gardner strain, and six squirrel monkeys received intravenous inoculation with SV40 777 strain. Eight of 10 monkeys received immunosuppression therapy, namely, cyclophosphamide subcutaneously either before or both before and after viral inoculation. The presence of viral infection was assessed by quantitative real-time PCR amplification of viral DNA from blood, urine, and 10 tissues. We found that squirrel monkeys were susceptible to infection with BKV, with high viral copy number detected in blood and viral genome detected in all tissues examined. BKV genome was detected in urine from only one monkey, while three monkeys manifested focal interstitial nephritis. BKV T antigen was expressed in renal peritubular capillary endothelial cells. By contrast, SV40 was detected at very low copy numbers in only a few tissues and was not detected in blood. We conclude that the squirrel monkey is a suitable animal for studies of experimental BKV infection and may facilitate studies of viral entry, pathogenesis, and therapy.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saimiri
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Infecciones Tumorales por Virus
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Replicación Viral
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Virus BK
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Virus 40 de los Simios
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Infecciones por Polyomavirus
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Modelos Animales de Enfermedad
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Virol
Año:
2005
Tipo del documento:
Article
Pais de publicación:
Estados Unidos