Improved distinction of factor V wild-type and factor V Leiden using a novel prothrombin-based activated protein C resistance assay.
Am J Clin Pathol
; 122(6): 836-42, 2004 Dec.
Article
en En
| MEDLINE
| ID: mdl-15539375
A new prothrombin-based activated protein C resistance (APC-R) test is described. In this method, the patient sample is prediluted in a plasma depleted of factor V (FV). A reagent containing APC and a specific activator of FV is added. After an incubation period, clotting is initiated by the addition of the FV-dependent prothrombin activator Noscarin. We analyzed 703 samples from patients undergoing thrombophilia screening. By using a predefined cutoff ratio of 2.5, 100% sensitivity and specificity for the detection of a factor V Leiden (FVL) mutation was found. With a cutoff ratio of 1.2, a complete but narrow distinction of FVL heterozygous (n = 192) and FVL homozygous samples (n = 27) was determined. No interference by the international normalized ratio, activated partial thromboplastin time (aPTT), protein S activity, fibrinogen and factor VIII (FVIII) levels, or lupus anticoagulant ratio was detected. The new prothrombin-based APC-R assay provides improved distinction of FV wild-type and FVL carriers compared with the aPTT-based method. By the use of an FV-dependent prothrombin activator, the assay is not influenced by FVIII concentration or lupus anticoagulants.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pruebas de Coagulación Sanguínea
/
Factor V
/
Protrombina
/
Proteína C
/
Deficiencia del Factor V
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Am J Clin Pathol
Año:
2004
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Reino Unido